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Thin layer chromatography (TLC) analysis of agar degradation by AgaML at different time points. Hydrolysis reactions were measured at pH 7.0 and 50 °C. Hydrolysates were taken at different incubation times and analyzed by TLC. Neoagarobiose (NA2), neoagarotetraose (NA4), neoagarohexaose (NA6), and <t>neoagarooctaose</t> <t>(NA8)</t> were used as standards.
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Thin layer chromatography (TLC) analysis of agar degradation by AgaML at different time points. Hydrolysis reactions were measured at pH 7.0 and 50 °C. Hydrolysates were taken at different incubation times and analyzed by TLC. Neoagarobiose (NA2), neoagarotetraose (NA4), neoagarohexaose (NA6), and neoagarooctaose (NA8) were used as standards.

Journal: International Journal of Molecular Sciences

Article Title: Isolation and Characterization of a Glycosyl Hydrolase Family 16 β-Agarase from a Mangrove Soil Metagenomic Library

doi: 10.3390/ijms17081360

Figure Lengend Snippet: Thin layer chromatography (TLC) analysis of agar degradation by AgaML at different time points. Hydrolysis reactions were measured at pH 7.0 and 50 °C. Hydrolysates were taken at different incubation times and analyzed by TLC. Neoagarobiose (NA2), neoagarotetraose (NA4), neoagarohexaose (NA6), and neoagarooctaose (NA8) were used as standards.

Article Snippet: The standards neoagarobiose (NA2), neoagarotetraose (NA4), neoagarohexaose (NA6), and neoagarooctaose (NA8) were from Qingdao BZ Oligo Biotech Co., Ltd., Qingdao, China.

Techniques: Thin Layer Chromatography, Incubation

Hydrolysis products of characterized agarases.

Journal: International Journal of Molecular Sciences

Article Title: Isolation and Characterization of a Glycosyl Hydrolase Family 16 β-Agarase from a Mangrove Soil Metagenomic Library

doi: 10.3390/ijms17081360

Figure Lengend Snippet: Hydrolysis products of characterized agarases.

Article Snippet: The standards neoagarobiose (NA2), neoagarotetraose (NA4), neoagarohexaose (NA6), and neoagarooctaose (NA8) were from Qingdao BZ Oligo Biotech Co., Ltd., Qingdao, China.

Techniques: